cPlot identifies the location and order of the sequencing reads by comparing the sequence to genetically close reference sequence in an effective way to visualize the assembly of short reads generated by NGS and rapid gene map construction. This application provides a web-based interface for the user for facile implementation, without the need for a dedicated computing environment. This application provides a web-based interface for the user for facile implementation, without the need for a dedicated computing environment. This file is licensed under the Creative Commons Attribution-Share Alike 4.0 International license.: You are free: to share to copy, distribute and transmit the work to remix to adapt the work Under the following conditions: attribution You must give appropriate credit, provide a link to the license, and indicate if changes were made. cPlot compares sequence similarity of reads by performing multiple read alignments, with FASTA format files as the input. We here devised cPlot software for read alignment of nucleotide sequences, with automated read alignment and position analysis, which allows visual assessment of the analysis results by the user.
#Cplot files manual
However, read alignment from vast amounts of genomic data from various organisms is challenging in that it involves repeated automatic and manual analysis steps.
In addition, it is essential to analyze the results of read alignments for a biologically meaningful inference. Reconstruction of short DNA sequence reads generated by next-generation sequencing requires a read alignment method that pieces together and reconstructs a reference genome. Advances in the next-generation sequencing technology have led to a dramatic decrease in read-generation cost and an increase in read output.
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